2022.08.18
Metabolome analysis service
Metabolism refers to chemical reactions that occur in the body that are indispensable for maintaining life. And, the ter…
Services
Metabolome analysis service
Metabolism refers to chemical reactions that occur in the body that are indispensable for maintaining life. And, the term metabolome is a combination of “metabolite” and “ome”, which means “all” in Greek. Literally translated, it means “the whole of metabolites”.
For example, the process of eating rice and breaking it down into glucose to produce energy is one type of metabolic reaction. So, metabolites are substances produced in the process of this chemical reaction, and there are many types including sugars, amino acids, lipids, nucleic acids, and other compounds that function as signal transmitters. In other words, metabolome analysis means “analyzing all metabolites that have wide ranging compositions”.
By analyzing all metabolites, it is possible to understand and monitor changes in the metabolic system inside the body. Knowledge of metabolic changes is fundamental to understanding life phenomena, for example, biological mechanisms that cause diseases can be understood at the biomolecular level.
Importantly, metabolome analysis, which “analyzes all metabolites,” requires extremely advanced technology to comprehensively analyze each metabolite.
NOSTER has collaborated with Professor Makoto Arita of Keio University Faculty of Pharmaceutical Sciences—an internationally renowned expert in lipoquality (lipid quality) research using lipid metabolome analysis technology— to develop and established unique mass spectrometry technology that comprehensively measures diet-derived gut microbiota lipid metabolites, thereby enabling analysis of specific metabolites.
NOSTER’s metabolome analysis technology provides metabolome analysis of metabolites produced by gut microbiota
There are 1,000 species and more than 100 trillion gut microbes in the human digestive tract, and human hosts construct a complex and diverse microbial ecosystem inside themselves while exchanging metabolites produced by the gut.
In a collaboration with Professor Jun Ogawa of Kyoto University, NOSTER has discovered that gut microbiota have lipid metabolism pathways that differ from those of humans, and that dietary lipids produce unique lipid metabolites.
In addition, with the aim of establishing a new therapeutic base, we have built an original lipid metabolite library that utilizes the lipid metabolism of gut bacteria using our unique intestinal bacterial culture and synthesis technology.
Applying this NOSTER original lipid metabolite library, we provide a unique metabolome analysis in the still unknown world of gut microbiota metabolites.
NOSTER’s Gut Bacterial Lipid Metabolite Library
Application areas
Wide range of areas related to lipids and microorganisms such as gut bacteria as described below.
Food sector
Development of supplements and exploration of new functions of fats and oils and fermented foods
Basic research
Analysis of metabolic mechanisms of gut bacteria
Medical fields
Elucidation of pathological mechanisms / Search and development of diagnostic markers/Evaluation of therapeutic effects
Others
Studies related to gut microbiota and disease
Overview
NOSTER specializes in providing metabolome analysis for gut microbiota lipid metabolites using linoleic acid, α-linolenic acid, and γ-linolenic acid contained in dietary oil as substrates.
| Target samples | feces, blood, food, culture medium, etc. |
|---|---|
| Main metabolites | Saturated / unsaturated fatty acids (linolenic acid-derived intestinal bacterial metabolites, α-linolenic acid-derived metabolites, γ-linolenic acid-derived metabolites, etc.) |
| Analysis operational procedure | Pretreatment (metabolite extraction) + separation analysis + data processing / analysis + report preparation / delivery |
| Analytical instrument | Liquid chromatograph mass spectrometer (LC-MS) |
| Report | Relative quantitative values, metabolic pathway map, etc. |
| Delivery time | 4-8 weeks |
| Cost | 200,000 yen per sample (Depending on the number of samples). We also have special price options for academia. Please contact us for more information. |
Optional plans
| Intestinal flora analysis | If the requested sample is feces, in addition to 16s metagenomic analysis by the next-generation sequencer, hybrid flora analysis that individually quantifies the number of bacteria per fecal weight is also available. |
|---|
NOSTER employs hybrid analysis of the gut microbiome that combines our unique in-house culture technology with gut microbe metagenomics analysis utilizing next-generation sequencing.
By combining the molecular biological approach to analyze the occupancy rate of each gut microbe with next-generation sequencing and our unique technology to culture anaerobic bacteria (Akkermansia, Bacteroides, Bifidobacterium, Blautia, Clostridium etc.) it is now possible to quantitively analyze the cell count per weight of feces. Notably, this hybrid, qualitative and quantitative analysis enables a much more detailed understanding of the microbiome that could not be achieved by existing metagenomic analysis alone.
About samples that are possible to analyze
Biological samples
| Measurement details | Feces, plasma, serum, breast milk |
|---|---|
| Required sample amount (approximate) |
Blood (plasma, serum) more than approx. 200 μL; feces of more than 300 mg. |
microbials
| Measurement details | Culture solution |
|---|---|
| Required sample amount (approximate) |
– |
food
| Measurement details | Processed foods, fresh foods |
|---|---|
| Required sample amount (approximate) |
– |
It may be possible to analyze samples not included in the list above. Please contact us for more information.
Metabolome analysis service image diagram
Targeted gut microbial lipid metabolites: * 1-4 is each analysis based on the total value of the two metabolites.
- LA Linoleic acid（cis-9-cis-12-octadecadienoic acid）
- HYA 10-hydroxy-cis-12-octadecenoic acid
- HYB 10-hydroxyoctadecanoic acid
- HYC 10-hydroxy-trans-11-octadecenoic acid
- KetoA 10-oxo-cis-12-octadecenoic acid
- KetoB 10-oxooctadecanoic acid
- KetoC 10-oxo-trans-11-octadecenoic acid
- 13(OH)LA 13-hydroxy-cis-9-octadecenoic acid
- 13(oxo)LA 13-oxo-cis-9-octadecenoic acid
- 10,13(diOH)LA 10,13-dihydroxyoctadecanoic acid
- CLA1 cis-9-trans-11-octadecadienoic acid ※1
- CLA2 trans-9-trans-11-octadecadienoic acid
- CLA3 trans-10-cis-12- octadecadienoic acid ※1
- OA Oleic Acid（cis-9-octadecenoic acid）
- ALA α-Linolenic acid（cis-9-cis-12-cis-15-octadecatrienoic acid）
- αHYA 10-hydroxy-cis-12-cis-15-octadecadienoic acid ※2
- αHYB 10-hydroxy-cis-15-octadecenoic acid
- αHYC 10-hydroxy-trans-11-cis-15-octadecadienoic acid ※2
- αKetoA 10-oxo-cis-12-cis-15-octadecadienoic acid ※3
- αKetoB 10-oxo-cis-15-octadecenoic acid
- αKetoC 10-oxo-trans-11-cis-15-octadecadienoic acid ※3
- 13(OH)ALA 13-hydroxy-cis-9-cis-15-octadecadienoic acid
- 13(oxo)ALA 13-oxo-cis-9-cis-15-octadecadienoic acid
- 10,13(diOH)ALA 10,13-dihydroxy-cis-15-octadecenoic acid
- GLA γ-Linolenic acid（cis-6-cis-9-cis-12-octadecatrienoic acid）
- γHYA 10-hydroxy-cis-6-cis-12-octadecadienoic acid ※4
- γHYB 10-hydroxy-cis-6-octadecenoic acid
- γHYC 10-hydroxy-cis-6-trans-11-octadecadienoic acid ※4
- γKetoA 10-oxo-cis-6-cis-12-octadecadienoic acid
- γKetoB 10-oxo-cis-6-octadecenoic acid
- γKetoC 10-oxo-cis-6-trans-11-octadecadienoic acid
- 13(OH)GLA 13-hydroxy-cis-6-cis-9-ctadecadienoic acid
- 13(oxo)GLA 13-oxo-cis-6-cis-9-octadecadienoic acid
- 10,13(diOH)GLA 10,13-dihydroxy-cis-6-octadecenoic acid
- RA Ricinoleic acid（12-hydroxy-cis-9-octadecenoic acid）
- KetoRA 12-oxo-cis-9-octadecenoic acid
- 12OH 12-hydroxyoctadecanoic acid
- 12oxo 12-oxooctadecanoic acid
- We plan to expand it in the future.
Example of an analysis report
Please note that the specifications are subject to change without notice.
Service flow
We will consult our customers about the details of the analysis method according to their research goals.
Please feel free to contact us.
1.Meeting
Establish the information necessary for measurement (research goals, type of sample, quantity, etc.)
2.Send sample to NOSTER
Transport by courier (refrigerated / frozen). Please ensure that the samples arrives on a working business day (Monday-Friday).
(Please inform the person in charge when shipping samples)
3.Storage
We will store the collected samples under appropriate conditions. (Refrigerator, freezer or ultra-low temperature freezer)
4.Pretreatment
Our staff with abundant experience in metabolome analysis will perform appropriate optimized pretreatment of the samples.
5.Measurement
We analyze the samples with our state of the art instruments under optimized conditions and methodology.
6.Analysis / Report
We will report peak area value comparison, relative comparison between groups, quantitative value, etc. according to the test system.
Precautions regarding analysis
- The results of the analysis can be used for research purposes only. They cannot be used for other purposes such as diagnosis.
- Please anonymize all personal information at the initial analysis request stage.
- We do not accept samples that may be infected, such as samples contaminated with pathogenic viruses.
- The analysis data will be stored for a year after the analysis is completed. It will be deleted after the storage period ends.
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